Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential. Molecular Cloning, Fourth Edition, by the celebrated founding author Joe Sambrook and new co-author, the distinguished HHMI investigator Michael Green, preserves the highly praised detail and clarity of previous editions and includes specific chapters and protocols commissioned for the book from expert practitioners at Yale, U Mass, Rockefeller University, Texas Tech, Cold Spring Harbor Laboratory, Washington University, and other leading institutions. The theoretical and historical underpinnings of techniques are prominent features of the presentation throughout, information that does much to help trouble-shoot experimental problems. For the fourth edition of this classic work, the content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Core chapters from the third edition have been revised to feature current strategies and approaches to the preparation and cloning of nucleic acids, gene transfer, and expression analysis. They are augmented by 12 new chapters which show how DNA, RNA, and proteins should be prepared, evaluated, and manipulated, and how data generation and analysis can be handled. The new content includes methods for studying interactions between cellular components, such as microarrays, next-generation sequencing technologies, RNA interference, and epigenetic analysis using DNA methylation techniques and chromatin immunoprecipitation. To make sense of the wealth of data produced by these techniques, a bioinformatics chapter describes the use of analytical tools for comparing sequences of genes and proteins and identifying common expression patterns among sets of genes. Please take a look at our book preview site at molecularcloning.com. Building on thirty years of trust, reliability, and authority, the fourth edition of Molecular Cloning is the new gold standard--the one indispensable molecular biology laboratory manual and reference source.

Español, Castellano Observaciones : ( INCLUYE TOMO 1 Y 3 ) FALTA EL TOMO 2 - TOMO 1 DESPRECINTADO CON LIGEROS ROCES POR LOS BORDES TOMO 1 PRECINTADO.

Molecular Biology of the Cell

For nearly a quarter century Molecular Biology of the Cell has been the leading cell biology textbook. This tradition continues with the new Fifth Edition, which has been completely revised and updated to describe our current, rapidly advancing understanding of cell biology. To list but a few examples, a large amount of new material is presented on epigenetics; stem cells; RNAi; comparative genomics; the latest cancer therapies; apoptosis (now its own separate chapter); and cell cycle control and the mechanics of M phase (now integrated into one chapter).

Ya reconocido como un clásico en el campo, este ejemplar reúne la experiencia de sus autores como docentes de grado y de posgrado, enfatizando determinados aspectos. En primer lugar, siendo la bioquimica un cuerpo de conocimientos compilado mediante la experiencia, han puesto el acento en como alcanzar dichas nociones, al generar la actitud critica necesaria para todo conocimiento cientifico. En segundo lugar tuvieron en cuenta la unidad de la vida y sus variantes a traves de la evolucion. El tercer aspecto consiste en que el proceso biologico esta organizado en una red elaborada e interdependiente. Por ultimo, se destaca el hecho de que la bioquimica tiene importantes consecuencias medicas. El texto se divide en cinco partes: I. Introduccion y Revision: un esbozo y analisis de las soluciones acuosas y de los elementos de termodinamica. II. Biomoleculas: con la descripcion de la estructura y funcion de las proteinas, acido nucleico, hidratos de carbono y grasas. III. Mecanismos de las enzimas activas: una introduccion a las propiedades, reaccion cinetica y mecanismos cataliticos de las enzimas. IV. Metabolismo: un estudio de como los organismos vivos sintetizan y degradan los hidratos de carbono, grasas, aminoacidos y nucleoticos, con enfasis en la generacion y consumo de energia. V....

This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging.

Como la cantidad de información en Biología seexpande cada vez más, se hace cada vez másnecesario que los libros de texto reflejen el vastoconocimiento científico en concisos principios yconceptos duraderos. Como en ediciones anteriores,Biologia Molecular de la Celula, Sexta Edicion,cumple este objetivo con un texto claro y excelentepuesta al dia con las ultimas investigaciones enel campo de la biologia celular y proporciona unsolido apoyo tanto al profesor como al estudiante.

This is the premier, single-source reference on redox biochemistry, a rapidly emerging field. This reference presents the basic principles and includes detailed chapters focusing on various aspects of five primary areas of redox biochemistry: antioxidant molecules and redox cofactors; antioxidant enzymes; redox regulation of physiological processes; pathological processes related to redox; and specialized methods. This is a go-to resource for professionals in pharmaceuticals, medicine, immunology, nutrition, and environmental fields and an excellent text for upper-level students.

Séptima edición, revisada y actualizada? La primera edición de Principios de Bioquímica deAlbert I. Lehninger, en 1970, supuso una auténtica revolución en la enseñanza de esta materia y creó un modelo que se ha mantenido fiel a lo largo de las sucesivas ediciones durante más de cuatro décadas.Con esta obra, que siempre ha sido cuidadosamente actualitzada, se han formado y desarrollado muchas generaciones de estudiantes, docentes e investigadores del mundo entero.? La bioquímica evoluciona de forma rápida y constante, y esta nueva edición, como suspredecesoras, reúne y ordena la información más actualizada y la pone a disposición tanto de los estudiantes que por primera vez se enfrentan a ella como de los expertos que la imparten, con un diseño que agiliza y facilita el estudio de la enorme cantidad de conocimiento científico que aporta. Cada uno de los capítulos ha sido minuciosamente revisado y puesto al día, de modo que esta séptima edición incluye todos los progresos que se han hecho en la disciplina: genómica, métodos bioquímicos, aplicaciones médicas y especial hincapié en el conocimiento del metabolismo y la evolución. Por último, proporciona innumerables herramientas y recursos didácticos orientados a alumnos y docentes.?

Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended.Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR.Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.

This book is meant to provide an in-depth understanding of the molecular biology of selenium and how this element makes its way into protein. Selenium is an essential trace element in the diet of humans and many other life forms. It plays an important role in preventing certain forms of cancer, heart disease and other cardiovascular and muscle disorders. Evidence points to numerous other health benefits including an indispensable function in development, male reproduction and the immune system. In addition, selenium serves as an antiviral agent and may delay the onset of AIDS in HIV positive patients and delay the aging process. The emphasis of this book is on the current status of the molecular biology of selenium. The importance of this element in human health is also highlighted. Features the many new discoveries have occurred since the 1st edition on Selenium was published in 2001 New investigators who have made important contributions to the field have been added as chapter authors